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Results verify previous considerations that the thermosensitive interval, when the embryo’s intercourse is directed to be male or female, doesn't match the center third of incubation. Moreover, utilizing the center third of incubation duration to approximate the thermosensitive period substantially decreases the accuracy of sex ratio estimates in natural nests. Identifying natural turtle hatchling sex ratios at nesting sites is challenging for a variety of causes, spanning morphological limitations to ethical points to an absence of understanding of the mechanisms that really direct embryonic sexual differentiation. Researchers from Florida Atlantic University’s Charles E. Schmidt College of Science have developed a first-of-its-variety approach that is minimally invasive and greatly enhances the power to measure neonate turtle sex ratios at population ranges throughout nesting websites worldwide. SRY recognizes conserved DNA sites in sex-specific promoters. This is the primary time that variations in intercourse-specific protein expression patterns have been recognized in blood samples of hatchlings with temperature-dependent sex determination.